Volume 5.25 | Jul 2

Mesenchymal Cell News 5.25 July 2, 2013
Mesenchymal Cell News
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Insufficient Stromal Support in MDS Results from Molecular and Functional Deficits of Mesenchymal Stromal Cells
Scientists showed that mesenchymal stromal cells (MSC) from patients across all MDS subtypes exhibit significantly reduced growth and proliferative capacities accompanied by premature replicative senescence. Osteogenic differentiation was significantly reduced in MDS-derived MSC indicated by cytochemical stainings and reduced expression of Osterix and Osteocalcin. [Leukemia] Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)
Human Mesenchymal Stem Cell-Replicative Senescence and Oxidative Stress Are Closely Linked to Aneuploidy
Scientists demonstrated that within the normal expansion period, human mesenchymal stem cells (hMSC) cultures show a high percentage of aneuploid cells that progressively increases until senescence. Despite this accumulation, they showed that in a heterogeneous culture the senescence-prone hMSC subpopulation has a lower proliferation potential and a higher incidence of aneuploidy than the non-senescent subpopulation. [Cell Death Dis] Full Article

Inhibition of Rac1 Promotes BMP-2-Induced Osteoblastic Differentiation
A previous study has demonstrated that the Ras homolog family-associated protein kinase (ROCK) inhibitor Y-27632 enhanced bone formation induced by recombinant human bone morphogenetic protein-2 (BMP-2) in vivo and in vitro. However, the effect of other Rho family members, such as Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division cycle 42, on bone formation remains unknown. In this study, scientists investigated whether Rac1 also participates in BMP-2-induced osteogenesis. Expression of a dominant-negative mutant of Rac1 enhanced BMP-2-induced osteoblastic differentiation in C2C12 cells, whereas a constitutively active mutant of Rac1 attenuated that effect. [Cell Death Dis] Full Article

GAPDH Is Critical for Superior Efficacy of Female Bone Marrow-Derived Mesenchymal Stem Cells on Pulmonary Hypertension
Researchers aimed to add new insight in gender differences of bone marrow-derived mesenchymal stem cells on therapy against pulmonary arterial hypertension and the underlying mechanism. In vitro experiments demonstrated superior function of female bone marrow-derived mesenchymal stem cells in cell proliferation, migration and [Ca2+]i kinetics. [Cardiovasc Res] Abstract

HIV Protease Inhibitors Induce Senescence and Alter Osteoblastic Potential of Human Bone Marrow Mesenchymal Stem Cells: Beneficial Effect of Pravastatin
In endothelial cells, some protease inhibitors (PIs) have been shown to induce the accumulation of farnesylated-prelamin-A, a biomarker of cell aging leading to cell senescence. Investigators hypothesized that these PIs could induce premature aging of osteoblast precursors, human bone marrow mesenchymal stem cells, and affect their capacity to differentiate into osteoblasts. [Aging Cell] Abstract

The Winged Helix Transcription Factor Foxa3 Regulates Adipocyte Differentiation and Depot-Selective Fat Tissue Expansion
The authors performed a siRNA screen of Forkhead-box genes in adipocytes and showed for the first time that the winged helix factor Foxa3 promotes adipocyte differentiation by cooperating with C/EBPß and d to transcriptionally induce PPAR? expression. [Mol Cell Biol] Abstract

Interleukin-6 Downregulation with Mesenchymal Stem Cell Differentiation Results in Loss of Immunoprivilege
Scientists investigated the influence of interleukin-6 (IL-6), a factor secreted by mesenchymal stem cell (MSC), on immune privilege after myogenic, endothelial and smooth muscle cell differentiation induced by 5-azacytidine, VEGF, and transforming growth factor-ß, respectively. Both RT-PCR and ELISA showed that myogenic differentiation of MSCs was associated with significant downregulation of IL-6 expression, which was also observed following endothelial and smooth muscle cell differentiation, indicating that IL-6 downregulation was dependent on differentiation but not cell phenotype. [J Cell Mol Med] Full Article

Prospective Purification of a Subpopulation of Human Synovial Mesenchymal Stem Cells with Enhanced Chondro-Osteogenic Potency
Scientists investigated marker sets for prospective purification of functionally distinct mesenchymal stem cell subsets. During culture expansion, the co-localization of CD39 within the CD73+ cell population identified a small cell subset that was maintained from passage 1 (P1) up to at least P12 in all donors tested. [Rheumatology] Abstract

Bone-Derived Mesenchymal Stromal Cells from HIV Transgenic Mice Exhibit Altered Proliferation, Differentiation Capacity and Paracrine Functions Along with Impaired Therapeutic Potential in Kidney Injury
Researchers evaluated cellular proliferation, differentiation and paracrine functions of mesenchymal stem cells (MSCs) isolated from compact bones of healthy control mice and Tg26 HIV-1 transgenic mice. They successfully isolated MSCs from healthy mice and Tg26 HIV-1 transgenic mice and found the latter expressed viral Nef, Vpu, NL4-3 and Vif genes. [Exp Cell Res] Abstract

Adipose-Derived Stem Cells Combined with Inorganic Bovine Bone in Calvarial Bone Healing in Type 2 Diabetic Rats
Scientists sought to investigate whether adipose-derived stem cells combined with inorganic bovine bone improve bone repair in calvarial vertical critical-sized defects in type 2 diabetic rats. The histological results showed an increase in new bone formation in the experimental groups compared with the control group. [J Periodontol] Abstract

New Methods for Inducing the Differentiation of Amniotic-Derived Mesenchymal Stem Cells into Motor Neuron Precursor Cells
Researchers investigated the differentiation of amniotic-derived mesenchymal stem cells into motor neuron precursor cells induced by a combination of extracellular matrix and multi-cell factors. [Tissue Cell] Abstract

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Actin Stress Fibre Subtypes in Mesenchymal-Migrating Cells
This review focuses on the current view of the molecular composition of actin stress fibre subtypes and how these fibre subtypes regulate mesenchymal cell migration. [Open Biol] Abstract

Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field.
Veterinarians Study Stem-Cell Therapy for Chronic Hepatitis
Veterinarians are looking at treatment of chronic hepatitis, or inflammation of the liver, with mesenchymal stem cells derived from excess fat tissue of healthy adult donors. These stem cells have the power to develop into other cell types, and may offer a new alternative in the fight against chronic hepatitis. [Colorado State University] Press Release

Mesoblast Receives Clearance to Begin Phase II Clinical Trial of Mesenchymal Precursor Cells in Diabetic Nephropathy
Mesoblast Limited announced that it has received approvals from Australian ethics committees to commence a Phase II trial evaluating a single intravenous infusion of its proprietary allogeneic or “off-the-shelf” adult Mesenchymal Precursor Cells in patients with type 2 diabetes and advanced kidney disease, or diabetic nephropathy. [Mesoblast Limited] Press Release

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NEW The European Cancer Congress 2013
September 27- October 1, 2013
Amsterdam, Netherlands

Visit our events page to see a complete list of events in the mesenchymal cell community.
NEW Postdoctoral Position – Cardiopulmonary Molecular Biology (Hannover Medical School)

NEW Two PhD Students and One Postdoc – Molecular Cell Biology and Hematopoiesis (Sanquin)

NEW PhD Positions – Tissue Engineering (Eight European Research Institutes)

Postdoctoral Fellow – Visualization of Gene Expression Promoting Mesenchymal Stem Cell Tropism (Johns Hopkins University School of Medicine)

Postdoctoral Researcher – Basic and Translational Hemato-Oncology (Josep Carreras Leukaemia Research Institute (IJC))

Director of Cell Processing Facility (S L Collins Associates, Inc.)

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